Illumina TruSeq RNA Access Library Prep Kit

Illumina TruSeq RNA Access Library Prep Kit - Set A (12 indexes, 48 samples)/RS-301-2001/1 Ea

產(chǎn)品編號： RS-301-2001

美  元  價： $7492.00

品       牌： Illumina

產(chǎn)       地： 美國

公       司： Illumina, Inc.

Product Highlights:

TruSeq RNA Access kits convert total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from FFPE (formalin-fixed, paraffin-embedded) tissues and other low-quality samples.

AffordABIlity and focus - isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth

High-quality data from difficult samples - optimized for sequencing RNA from degraded samples, including FFPE tissues

Samples with limited starting material - greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity

TruSeq RNA Access Kit generates RNA-Seq libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions maximizes discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time ~2 Days

Hands-on Time ~11 hours

Input Quantity 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples

Content Specifications Captures the coding transcriptome/RNA exome

Mechanism of Action Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails.

Multiplexing Up to 24 unique indexes and 4-plex pre-enrichment pooling

System Compatibility NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Novel Transcripts,Transcript Variants

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Method mRNA Sequencing,Exome Sequencing

Species Category Human

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